ImageXpress Micro Confocal High-Content Imaging System
Leszek Kotula, M.D., Ph.D., 315-464-1690, email@example.com
This versatile imaging system features a unique confocal technology, which allows you to explore more physiologically, relevant, complex three dimensional models, including spheroids, tissues, and whole organisms and to generate publication quality images at high throughput for samples in slides or one to 1536‑well microplates. The key feature of the device is on board robotic fluidic head, including the following specifications: automated liquid handling for compound/media addition; ability to perform well washing using disposable pipette tips; add, wash, and dispense into user configurable patterns; fully customized workflow through scripting; single-channel pipettor with dispense volumes from 3 µL to 200 µL (±1 µL ±5%); supports 96- or 384-well format pipette tips; holds two temperature-controlled plates for compound addition or media exchange; 3D analysis tools are integrated into acquisition and analysis software and are able to measure volumes, diameters, and distances in 3 dimensions. In addition, software runs on 8 parallel processors for fast, multi-threaded analysis.
Key capabilities of the microscope:
- Select a confocal geometry optimal for your assay and throughput needs
- Achieve excellent image quality without sacrificing throughput via our unique optical path technology
- Acquire statistically relevant data quickly with an advanced scientific CMOS detector, enabling >3 log dynamic range
- Large field of view enables whole-well imaging
- Expand your research capabilities with transmitted light, liquid handling, and environmental options
- Capture an entire well of a 384‑well plate with a single image at 4X magnification
- Capture four wells of a 1536- well plate in a single image at 4X magnification
- Throughput of >160K wells/day confocal, >200K wells/day widefield 2 ImageXpress
Rules of operation
The ImageXpress Micro confocal is open to all investigators in the University, we have to enforce some rules to insure every investigator has equal access to our equipments:
- All users must be trained before they are allowed to use the microscopes. After training, an account will be set up for reserving time and using the microscope.
- All users must sign up on the online calendar to reserve time before use of the microscopes.
- Reservation policies:
- No reservation more than 2 weeks in advance
- The reservation times may separate to two categories:
- Regular working slots (8am to 5pm, Monday to Friday)
- Off-hour slots (weekend and weekdays before 8am or after 5pm).
- During a regular working slot one user can reserve an instrument for max 4 hours per day and 8 hours per week; however this can be combined with an off-hour slot where the reservation is not limited.
- If you are unable to use the microscope that you have reserved, Please delete your reserved time on the calendar as far ahead as possible so others can see the time is available.
- If you finished before the end of your reserved time or cancel on the day of your reserved session, please inform the next or previous person in line so that he/she can start earlier or continue longer. If you are the last user of the day and you cancel your reserve within 24 hours, you are responsible for ensuring the microscope is turned off. Make sure to inform the user before you to shut down the system after finish, otherwise he might leave the microscope on for you.
- Using the microscope:
- Please start your session punctually. Repeat "No-shows" will lose their sign-up privileges.
- Please check the online booking calendar at the end of your session to facilitate last minute changes and scheduling updates. Leave the system on if someone is reserved next within two hours; keep the laser in standby whenever possible to prolong their life.
- Users must clean the microscope objectives they used and dispose of garbage when finish. If you bring any live specimens or potentially harmful samples, please dispose of them in your lab.
- The last user of the evening is responsible for shutting down the microscope system.
- You are responsible for your data. The system computers are for temporary image storage. Please save your data on the data hard drive of the computer and transfer your image data as soon as possible after acquisition. Image files left on the computer hard drives will be removed in a period of time.
- Users are required to report any problems or damages during usage of the microscope.
- No food or drink is allowed in the microscope rooms.
- The hours billed are based on your computer log off. Remember to log off to end the billing session.
- All users must record system usage on the paper log at each microscope.
- Minimum time billed is 1 hr
- Each imaging session will be billed by the larger of either the time reserved or hours used in 1 hr increments (i.e., all time is round up to a full hour)
- Technical support is billed in 1 min increments and added to the cost/hr for the usage
- The annual power user fee has been replaced by the quarterly discount. There is now a quarterly cap on the cost of laser use. Other fees (training, technical support etc) are billed in addition to laser use and are not discounted.
If you fail to show up for time you have scheduled on the calendar, you will be billed for the amount of time scheduled.
Removal (cancellation) or modification of time scheduled on the calendar must be done at least 24 hr before the time you were scheduled for or you will be billed for the amount of time originally scheduled. (Note the calendar keeps electronic record of all entries and modifications).
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