766 Irving Avenue
Syracuse, NY 13210
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Phone: 315 464-7691
Name: Lisa Phelps, Flow Core Operator
The SUNY Upstate Medical University Research Flow Core is available to all researchers at SUNY and surrounding institutions. We offer four flow cytometers and training for all researchers in the fundamentals of flow cytometry. A full-time flow core operator is available for assistance and guidance during normal business hours. The flow core operator is responsible the maintenance and calibration of the instruments.
We have three cell analyzers in our facility. Samples analyzed on the BD LSRII, BD LSRFortessa, or Cytek Aurora cannot be reclaimed. Our goal is to provide each user with the skills to operate the cell analyzers independently. All users are trained prior to using the instruments. Training covers the fundamentals of flow cytometry theory, start-up and shut-down procedures, file acquisition, and trouble-shooting of common operator issues. After the initial training, users are shadowed until their proficiency is demonstrated. They may then use the instruments independently. The laser configuration of the 3 instruments varies. The LSRII has UV (355nm), Blue (488nm), Yellow Green (561nm) and Red (640nm) lasers capable of analyzing experiments with up to 13 colors. The LSRFortessa has Violet (405nm), Blue (488nm), and Red (640nm) lasers capable of analyzing experiments with up to 16 colors. The Aurora has UV (355nm), Violet (405nm), Blue (488nm), Yellow Green (561nm) and Red (640nm) lasers capable of analyzing experiments with up to 35 colors. See the links below for instrument-specific configurations.
The flow core also has a BD FACSAriaIII cell sorter. Subpopulations of cells sorted on the AriaIII can be collected into 12x75 mm or 15ml tubes, plates (6 well to 384 well), or slides. The FACSAriaIII has Violet (405nm), Blue (488nm), Yellow Green (561nm), and Red (640nm) lasers capable or sorting experiments with up to 14 colors. See the link below for the FACSAriaIII laser configuration. The instrument has a BioBubble enclosure installed to allow sorting of BL-2 materials. The Flow Core Operator is the sole operator of the sorter.
- Becton Dickinson LSRII Cell Analyzer
- Becton Dickinson Fortessa Cell Analyzer
- Becton Dickinson FACS Aria III Cell Sorter
- Cytek Aurora Spectral Signature