Core Facility for Proteomics & Mass Spectrometry
766 Irving Avenue
Syracuse, NY 13210
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Phone: 315 464-8744
Name: Ebbing de Jong
The Upstate Proteomics & Mass Spectrometry Core Facility offers Upstate (and external) researchers cutting edge technology to support research projects involving proteomics or metabolomics applications.
The state-of-the-art Orbitrap mass spectrometer series was installed in November 2018. The Fusion Lumos performs highly advanced scan functions for quantitative proteomics studies, as well as diverse fragmentation modes for the analysis of post-translational modifications. Its high scan speed and sensitivity result in significantly greater proteome coverage.
A different type of mass spectrometer is more suitable when measuring known molecules in a sample: a triple quadrupole. Ideal for targeted analyses, the Thermo Quantis excels at quantifying small molecules as well as peptides in a range of sample matrices. This instrument features short analyses for a quick turnaround time of customers’ samples.
Supported applications include (but are not limited to):
- protein identification from SDS-PAGE gels
- protein molecular mass determination
- analysis of protein posttranslational modifications
- comparative quantification of protein expression levels in cell culture and tissue samples
- identification and quantification of small molecules and metabolites
Bruker Microflex SH/LT
This MALDI-TOF instrument is ideal for MW confirmation or determination for medium to large molecules: proteins, peptides, polymers and oligonucleotides. As there is no chromatographic separation inline with the instrument, samples should be of relatively low complexity and mostly free of salts, buffers and detergents. Interested users may be trained to use this instrument, or submit samples for analysis by core facility personnel.