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Antibodies

Immunofluorescence staining

Here is a list of some antibodies we use for kidney samples, with information on optimal fixation (with thanks to our SURF student Sam Harris)

List of antibodies and information on their use
Antibody name IHC-cryo IHC-paraffin Western blot Cell staining Notes
ZO-1, Thermo Fisher, cat#1A12

Mouse AB; works with PFA or MeOH/acetone fixation

PECAM-1 (endothelial marker), BD, cat#550274

Rat AB, works much better with MeOH/acetone fixation
Synaptopodin G1D4 (podocyte marker), GeneTex cat#GTX39067 Mouse AB; works with PFA or MeOH/acetone fixation
Nephrin (podocyte marker), Fitzgerald, cat#2OR-NP002 Guinea pig AB; a faint lower MW band in mouse kidney lysate, with a strong band of >100 kDa as expected
WT-1 (podocyte nuclear marker), Abcam, cat# ab89901 Rabbit AB
Smooth muscle actin (mesangial cell and smooth muscle cell marker), Sigma, cat# A2547 Mouse AB; works with PFA or MeOH/acetone fixation
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