Antibodies
Here is a list of some antibodies we use for kidney samples, with information on optimal fixation (with thanks to our SURF student Sam Harris)
| Antibody name | IHC-cryo | IHC-paraffin | Western blot | Cell staining | Notes |
| ZO-1, Thermo Fisher, cat#1A12 | √ |
Mouse AB; works with PFA or MeOH/acetone fixation |
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PECAM-1 (endothelial marker), BD, cat#550274 |
√ | Rat AB, works much better with MeOH/acetone fixation | |||
| Synaptopodin G1D4 (podocyte marker), GeneTex cat#GTX39067 | √ | Mouse AB; works with PFA or MeOH/acetone fixation | |||
| Nephrin (podocyte marker), Fitzgerald, cat#2OR-NP002 | √ | √ | Guinea pig AB; a faint lower MW band in mouse kidney lysate, with a strong band of >100 kDa as expected | ||
| WT-1 (podocyte nuclear marker), Abcam, cat# ab89901 | √ | Rabbit AB | |||
| Smooth muscle actin (mesangial cell and smooth muscle cell marker), Sigma, cat# A2547 | √ | Mouse AB; works with PFA or MeOH/acetone fixation |