Protocols
This page will provide protocols which have been developed, or just modified from standard protocols, in our laboratory. It is by no means a comprehensive listing of all of the protocols we use.
Yeast Biology
- Actin Capture Assay (D. Amberg)
- Colony PCR (D. Amberg)
- Immunofluorescence (with Methanol) (D. Amberg)
- Immunofluorescence (without methanol) (D. Amberg)
- Rhodamine-Phalloidin/Calcofluor Staining (D. Amberg)
- "Smash and Grab" Recovery of Plasmids (D. Amberg)
- Isolation of Yeast Genomic DNA (D. Amberg)
- Isolation of Yeast Protein (D. Amberg)
- Yeast Transformation (D. Amberg)
General Techniques
- Gene Disruption by Fusion PCR (E. Beasley and D. Amberg)
- Sequencing Template Preparation (D. Amberg)