This page will provide protocols which have been developed, or just modified from standard protocols, in our laboratory. It is by no means a comprehensive listing of all of the protocols we use.

Yeast Biology

• Actin Capture Assay (D. Amberg)
• Colony PCR (D. Amberg)
• Immunofluorescence (with Methanol) (D. Amberg)
• Immunofluorescence (without methanol) (D. Amberg)
• Rhodamine-Phalloidin/Calcofluor Staining (D. Amberg)
• "Smash and Grab" Recovery of Plasmids (D. Amberg)
• Isolation of Yeast Genomic DNA (D. Amberg)
• Isolation of Yeast Protein (D. Amberg)
• Yeast Transformation (D. Amberg)

General Techniques

• Gene Disruption by Fusion PCR (E. Beasley and D. Amberg)
• Sequencing Template Preparation (D. Amberg)