AffymetrixGeneChip© Core Facility (3285 Weiskotten Hall)
The central technology of SUNYMAC is an Affymetrix Microarray Suite that includes two Affymetrix hybridization ovens, three microfluidics processing stations, the latest Affyetrix GeneChip 7G/4C scanner with Autoloader, and two data analysis workstations.
Roche LightCycler 480 Real Time PCR / High Resolution Melt Curve Instrument
Investigators needing high-throughput real time PCR validation of microarray findings, or genotyping of individual SNPs in hundreds of samples at a time, can utilize the state of the art 384 well plate format that this instrument provides.
Bio-Rad Bio-Plex 200 System with HTF
The Bio-Plex suspension array system utilizes Luminex xMAP technology to permit the multiplexing of up to 100 different assays within a single sample.
Bio-Plex Pro II Wash Station
This instrument is a microplate wash station for magnetic and polystyrene bead based assays.
Bio-Plex Pro wash stations eliminate manual wash steps from Bio-Plex assays, making Bio-Plex assays as easy as ELISAs.
This instrument provides highly-consistent automated preparation of DNA, RNA, protein, and plasmid preps, and is recommended for large scale studies using more than 24 samples.
Leica Laser Microdissection Apparatus
The analysis of gene expression in localized population of cells or specific anatomical structures requires the ability to harvest these regions of interest with precision. The AMD Leica Laser Microdissection instrument makes this possible. Training is provided free of charge, and access to the instrument is available by scheduling (at $15/hour).
BioTek MicroFlo Select Dispenser
Consistent results for any high-throughout molecular biology protocol are now more easily obtained through the use of an automated fluid handling system, that can dispense extremely accurate volumes from 1 uL - 2.5 mL to an entire plate or rack of tubes in a matter of seconds.
Agilent Technologies Bioanalyzer
In order to assess the quality and quantity of minute amounts of starting material from laser microdissected cells, SUNYMAC also maintains an Agilent Technologies Bioanalyzer that is capable of assessing RNA quality from as little as 250 pg of total RNA. Highly-accurate sizing and quantitation of DNA and proteins are also possible.
BioTek Synergy 2 Microplate Reader
This high-throughput microplate reader can perform rapidly analyse full range UV spectrophotometry, fluorescence, and luminescence. It is also equipped with automated dual injectors and an incubator, to permit numerous cell-based assays (e.g., dual-wavelength ELISAs).
Data Analysis Workstations
The software needed to perform gene expression, genotyping, and real-time PCR data analyses are available free of charge to interested researchers on several dedicated high-end PC workstations.
Bio-Rad Tetrad DNA Engine
Increase your PCR throughput by reserving time on the Core Facility's Bio-Rad Tetrad instrument.
For questions or to drop off samples contact:Contact: Karen Gentile, Technical and Administrative Director of the Core
Location: Upstate Medical University, Neuroscience
Weiskotten Hall 3285
750 East Adams Street, Syracuse, NY 13210
Phone: 315 464-3206