Welcome to SUNYMAC
SUNYMAC is a core facility of the State University of New York Upstate Medical
University in Syracuse, NY. It is a full service microarray processing core
that specializes in high-throughput gene expression and genotyping analysis
using Affymetrix
GeneChip©
 microarrays and real-time quantitative PCR. SUNYMAC is also a data analysis
facility and a technical resource for targeted analysis of biological samples,
using laser microdissection. SUNYMAC was established in October 2002 and has
grown to a client base of more than 50 investigators, with current throughput
estimates of 1,500 - 2,000 samples per year. SUNYMAC is affiliated with both
the AMDeC
consortium and Affymetrix Core Lab Program.
Resources
The central technology of SUNYMAC is an AffymetrixMicroarray Suite, that includes
an Affymetrix hybridization oven, two microfluidics processing stations, the
latest Affyetrix GeneChip 7G/4C scanner with Autoloader, and two data analysis
workstations.
Investigators needing high-throughput real time PCR validation of microarray
findings, or genotyping of individual SNPs in hundreds of samples at a time,
can utilize the state of the art 384 well plate format that this instrument
provides. Training and instrument access are provided free of charge.
For lower-throughput validation studies of gene expression findings or genotyping
of single nucleotide polymorphisms in a 96 well plate format. Training and instrument
access are provided free of charge.
This instrument provides highly-consistent automated preparation of DNA, RNA,
protein, and plasmid preps, and is recommended for large scale studies using
more than 24 samples. Training and instrument access are provided free of charge.
The analysis of gene expression in localized population of cells or specific
anatomical structures requires the ability to harvest these regions of interest
with precision. The AMD Leica Laser Microdissection instrument makes this possible.
Training is provided free of charge, and access to the instrument is available
by scheduling (at $15/hour).
Consistent results for any high-throughout molecular biology protocol are now
more easily obtained through the use of an automated fluid handling system,
that can dispense extremely accurate volumes from 1 uL - 2.5 mL to an entire
plate or rack of tubes in a matter of seconds. Training and instrument access
are provided free of charge.
In order to assess the quality and quantity of minute amounts of starting material
from laser microdissected cells, SUNYMAC also maintains an Agilent Technologies
Bioanalyzer that is capable of assessing RNA quality from as little as 250 pg
of total RNA. Highly-accurate sizing and quantitation of DNA and proteins are
also possible.
This high-throughput microplate reader can perform rapidly analyse full range
UV spectrophotometry, fluorescence, and luminescence. It is also equipped with
automated dual injectors and an incubator, to permit numerous cell-based assays
(e.g., dual-wavelength ELISAs). Training and instrument access are provided
free of charge.
Data Analysis Workstations
The software needed to perform gene expression, genotyping, and real-time PCR
data analyses are available free of charge to interested researchers on several
dedicated high-end PC workstations.
For whole-mount and section-based semi-automated processing of tissue using
cRNA riboprobes or cDNA oligonucleotide probes, the Core Facility offers SUNY
Upstate researchers access and training on the Bio Lane HTI Instrument.
Increase your PCR throughput by reserving time on the Core Facility's Bio-Rad
Tetrad instrument. Access and training free of charge.
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