Welcome to SUNYMAC
SUNYMAC is a core facility of the State University of New York Upstate Medical
University in Syracuse, NY. It is a full service microarray processing core
that specializes in high-throughput gene expression and genotyping analysis
using Affymetrix
GeneChip©
 microarrays and real-time quantitative PCR. SUNYMAC is also a data analysis
facility and a technical resource for targeted analysis of biological samples,
using laser microdissection. SUNYMAC was established in October 2002 and has
grown to a client base of more than 50 investigators, with current throughput
estimates of 1,500 - 2,000 samples per year. SUNYMAC is affiliated with both
the AMDeC
consortium and Affymetrix Core Lab Program.
Resources
The central technology of SUNYMAC is an Affymetrix Microarray Suite that includes
two Affymetrix hybridization ovens, three microfluidics processing stations, the
latest Affyetrix GeneChip 7G/4C scanner with Autoloader, and two data analysis
workstations.
Investigators needing high-throughput real time PCR validation of microarray
findings, or genotyping of individual SNPs in hundreds of samples at a time,
can utilize the state of the art 384 well plate format that this instrument
provides.
The Bio-Plex suspension array system utilizes Luminex xMAP technology to permit the multiplexing of up to 100 different assays within a single sample.
This instrument is a microplate wash station for magnetic and polystyrene bead based assays.
Bio-Plex Pro wash stations eliminate manual wash steps from Bio-Plex assays, making Bio-Plex assays as easy as ELISAs.
This instrument provides highly-consistent automated preparation of DNA, RNA,
protein, and plasmid preps, and is recommended for large scale studies using
more than 24 samples.
The analysis of gene expression in localized population of cells or specific
anatomical structures requires the ability to harvest these regions of interest
with precision. The AMD Leica Laser Microdissection instrument makes this possible.
Training is provided free of charge, and access to the instrument is available
by scheduling (at $15/hour).
Consistent results for any high-throughout molecular biology protocol are now
more easily obtained through the use of an automated fluid handling system,
that can dispense extremely accurate volumes from 1 uL - 2.5 mL to an entire
plate or rack of tubes in a matter of seconds.
In order to assess the quality and quantity of minute amounts of starting material
from laser microdissected cells, SUNYMAC also maintains an Agilent Technologies
Bioanalyzer that is capable of assessing RNA quality from as little as 250 pg
of total RNA. Highly-accurate sizing and quantitation of DNA and proteins are
also possible.
This high-throughput microplate reader can perform rapidly analyse full range
UV spectrophotometry, fluorescence, and luminescence. It is also equipped with
automated dual injectors and an incubator, to permit numerous cell-based assays
(e.g., dual-wavelength ELISAs).
Data Analysis Workstations
The software needed to perform gene expression, genotyping, and real-time PCR
data analyses are available free of charge to interested researchers on several
dedicated high-end PC workstations.
Increase your PCR throughput by reserving time on the Core Facility's Bio-Rad
Tetrad instrument.
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