Confocal & Two-Photon Imaging Core
This core facility enables neuroscientists to visualize the structure of individual neurons and their activity in the living organism and has potential applications in many other research areas (such as microbiology, molecular biology, ophthalmology, etc.) for the study of 3D structure and process dynamics.
The core contains:
- Zeiss LSM510 scanning confocal/two-photon microscope system
- Coherent Chameleon tunable phased NIR laser for two-photon excitation
- Newport vibration isolation optical table
- A separate computer with Zeiss software for image analysis
The microscope system consists of two microscopes (inverted and upright) that share a portable scanhead with multiphoton excitation imaging and spectral detector capabilities. Three independent simultaneous confocal channels for reflection and fluorescence are available as well as fourth channel for transmission (DIC) imaging.
This system employs a PC-based user interface to control the microscope, laser excitation, scanning and image acquisition, and is capable of digitally capturing up to 12-bit 2,048x2,048 images, while the LSM 5 Software controls image data acquisition, presentation, processing, and archiving, with 3D image reconstruction and multiple time series features also available.
For more information and to discuss usage, contact: Arvydas Matiukas, PhD
Phone 315 464-7997
matiukaa@upstate.edu
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