DNA Sequencing

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Core: DNA Sequencing
Core Director: Antony Shrimpton, PhD
Core Contact: Vicki Lyle
Phone Number: 315 464-6544
Location: 4840 University Hospital
Email: lylev@upstate.edu
Web Site: DNA Sequencing

Instrumentation in Core:

  • ABI 3130XL Genetic Analyzer

Brief Description of Core:  Provides automated DNA sequencing of single stranded and double stranded DNA samples from purified plasmids and PCR products. Samples are sequenced using the BIG DYE Terminator Version 3.1 Cycle Sequencing Kit from Applied Biosystems and are run on an ABI 3130XL Genetic Analyzer. This automated sequencer uses a four color fluorescent dye system and typically provides 600-1000 bases of usable sequence data per reaction. Our normal turn-around time is one working day after receipt of samples. 

Core Fee Schedule:  The fee for DNA Sequencing is currently $10.00 / reaction run for SUNY Upstate Medical University customers and $11.00 for all outside customers.  One reaction consists of one DNA template (customer-supplied plasmid DNA or PCR product) being sequenced with one primer.  A discounted rate of $9.00 / reaction for in-house SUNY UMC customers and $10.00 / reaction for outside customers is also offered when one principal investigator brings 50 or more samples as a single batch to be sequenced.  The Core now carries 12 stock primers at no additional charge (M13 Forward, M13 Reverse, T7 Promoter, T7T Terminator, T3 Promoter, SP6 Promoter, SP6 Upstream, BGH Reverse, PGEX3’, PGEX5’, EGFP-C and EGFP-N).

How to Access Core Services:  Walk in; open Monday through Friday, 9:00 am to 5:00 pm for your convenience.