Flow Cytometry

Flow Cytometry, Research
Weiskotten Hall
Rm. 2321,
766 Irving Avenue
Syracuse, NY 13210
Map & directions
Phone: 315 464-7691
Name: Lisa Phelps, Flow Core Operator
Email: Phelpsl@upstate.edu

The SUNY Upstate Medical University Research Flow Core is available to all researchers at SUNY and surrounding institutions. We offer three flow cytometers and training for all researchers in the fundamentals of flow cytometry.

Our goal is to provide each user with the skills to operate the cell analyzers independently. All users are trained prior to using the instruments. Training covers the fundamentals of flow cytometry theory, start-up and shut-down procedures, basic acquisition using the BD DIVA software, and trouble-shooting of common operator issues.

Operator with flow cytometry machine.After the initial training, users are shadowed until their proficiency is demonstrated. They may then use the instruments independently. A full-time flow core operator is available for assistance and guidance during normal business hours. The flow core operator is responsible for the maintenance and calibration of the instruments.

We have two cell analyzers in our facility. Samples analyzed on the BD LSRII or BD LSRFortessa cannot be reclaimed. The laser configuration of the 2 instruments varies slightly. The LSRII has UV (355nm), Blue (488nm), Yellow Green (561nm) and Red (640nm) lasers capable of analyzing experiments with up to 13 colors. The LSRFortessa has Violet (405nm), Blue (488nm), and Red (640nm) lasers capable of analyzing experiment with up to 16 colors. See the links below for instrument-specific configurations.

The flow core also has a BD FACSAriaIII cell sorter. Subpopulations of cells sorted on the AriaIII can be collected into 12x75 or 15ml tubes, plates (6 well to 384 well), or slides for further experiments. The FACSAriaIII has Violet (405nm), Blue (488nm), Yellow Green (561nm), and Red (640nm) lasers capable or sorting experiments with up to 14 colors. See the link below for the FACSAriaIII laser configuration. The instrument has a BioBubble enclosure installed to allow sorting of BL-2 materials. The Flow Core Operator is the sole operator of the sorter.

Laser Configurations

Resources

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