Section: Molecular Pathology

Duration: 2 weeks

Goals & Objectives

Statement of Goals: The goal of the Molecular Pathology rotation is to provide an opportunity to learn the principles and applications of methods used in clinical testing involving molecular biology. To be exposed to the process of applying and interpreting data generated by molecular tests including potential pitfalls and other limitations.

Become familiar with and understand the basis for all molecular techniques used in clinical laboratory testing. Including:

DNA isolation/preparation
Restriction endonuclease digestion of DNA
Gel electrophoresis
Hybridization theory (Southern blot, dot blot, etc.)
Polymerase Chain Reaction (PCR) theory and technique

Residents will have the opportunity to perform hands-on molecular testing involving PCR amplification and detection of mutations.

Understand the general approach for implementing and validating molecular assays for clinical service work.
Understand the principles and techniques involved in molecular genetics, molecular oncology and molecular detection of infectious diseases.

Duties & Responsiblities

Participate in the interpretation and reporting of various assays with the molecular biologist.
At present, the Molecular Pathology section has responsibilities to perform testing in molecular genetics and molecular oncology and to perform research and development in molecular genetics, oncology and infectious diseases.

Evaluation:Global Rating of Live or Recorded Performance: A rater judges general categories of ability (patient care skills, medical knowledge, interpersonal and communication skills) and the ratings are completed retrospectively based on general impressions collected over a period of time (end of rotation) derived from multiple sources of information (direct observations or interactions); input from other faculty, lab technicians and residents and review of work products or written materials.