doublehelix

DNA Double Helix

dnapipette

DNA in Pipette

cellwdna

Cell with DNA

abi

ABI 3130XL Genetic Analyzer

Available Stock Sequencing Primers

The Core Facility for DNA Sequencing now has 12 stock primers available that we will provide for sequencing:

M13 Forward(-20) 5' - GTA AAA CGA CGG CCA GT - 3'
M13 Reverse(-27) 5' - CAG GAA ACA GCT ATG AC - 3'
T3 Promoter 5' - AAT TAA CCC TCA CTA AAG GG - 3'
T7 Promoter 5' - TAA TAC GAC TCA CTA TAG GG - 3'
T7 Terminator 5' - GCT AGT TAT TGC TCA GCG G - 3'
SP6 Promoter 5' - TAC GAT TTA GGT GAC ACT ATA G - 3'
SP6 Upstream 5' - ATT TAG GTG ACA CTA TAG - 3'
BGH Reverse 5' - TAG AAG GCA CAG TCG AGG - 3'
PGEX3' 5' - CCG GGA GCT GCA TGT GTC AGA GG - 3'  
PGEX5' 5' - GGG CTG GCA AGC CAC GTT TGG TG - 3'
EGFP-C 5' - CAT GGT CCT GCT GGA GTT CGT G - 3'
EGFP-N 5' - CGT CGC CGT CCA GCT CGA CCA G - 3'