RESEARCH PROFILE
original: wild-type paxillin cells
rollover: paxillin lacking LD4 cells

These cells were scratch-wounded followed by fixation 12-hours post-wounding. Tubulin (Green) and alpha-mannosidase II (Red) were labeled to note cell polarization and Golgi orientation. Cells expressing paxillin lacking LD4 are unable to reorient the Golgi towards the wound edge. From the lab of Christopher Turner, PhD.

Check out our Research Gallery

In this movie, localization of GFP-tagged myo1e (green,) and RFP-tagged clathrin (red) at the site of endocytosis was examined using TIRF microscopy.

Experimental Methods

TIRF

These are some of the experimental techniques commonly used in the lab:

  • Live cell imaging and analysis of dynamics of fluorescently tagged proteins.
  • Physiological characterization of renal functions in mice.
  • Mouse model of type I diabetes (streptozotocine-induced diabetes).
  • Histology, immunohistochemistry, and electron microscopic analysis of animal tissue samples.
  • Measurements of cell migration rates using wound-healing model and Boyden chamber migration assays.
  • Use of adenoviral vectors for recombinant protein expression and protein knockdown.
  • Protein-protein interaction assays (pulldowns, immunoprecipitation, yeast two-hybrid).
This time-lapse movie of wound healing was collected after scraping a monolayer of epithelial cells with a needle and allowing cells to migrate into the wound.