Richard D Veenstra, PhD
Research Programs and Affiliations
- Biomedical Sciences Program
- Cancer Research Institute
- Neuroscience Program
- Research Pillars
Education & Fellowships
- PhD: University of Iowa, 1983
- Regulation of connexin-specific-gap-junctions; gap junction channel biophysics.
HealthLinkOnAir Radio Interview1/24/13 "Go Red for Women" initiative aims to increase public awareness about heart disease in women
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The contractile function of the heart is controlled by the electrical activity initiated in the pacemaker sinoatrial node and conducted throughout the heart. An electrical impulse, called an action potential, is generated by specific ion channels that are directly regulated by membrane voltage or ionic blockade. The rapid transmission of this 1/10th of a volt signal is accomplished by gap junctions, a type of intercellular junction which forms a tunnel-like channel. Gap junctions are typically open at rest and, hence, do not require voltage-dependent activation. Little is known about how gap channel proteins, called connexins, conduct electric current and how this current flow is regulated by physiological or pathophysiological (disease) conditions.
By producing site-directed mutations in the two major cardiac gap junction proteins, connexin43 (Cx43) and connexin40 (Cx40), we are examining the molecular basis for the selective electrical conductance and molecular permeability properties of cardiac gap junctions. We hope to make structural inferences about gap junction channel pore structure by observing how endogenous polyamines, small polybasic molecules derived from amino acids such as spermine, block Cx40 and three other connexin gap junctions while having no effect on Cx43 and the majority of other 20 mammalian connexin-specific gap junctions. Intracellular calcium elicits myocardial contraction and, at least under pathophysiological conditions, can shut down gap junction communication. Recently published observations provide new insights as to how this is effected and we are studying how the (patho)physiological regulation of cardiac, lens, and liver gap junctions may be altered by naturally occurring mutations. We are also examining the mechanisms by which human atrial fibrillation mutations in Cx40 or Cx43 alter cardiac gap junction function to produce cardiac arrhythmias or other human diseases.
There is no known clinical therapeutic pharmacology for gap junctions despite their importance to the conduction of the heartbeat for every second of life. We have investigated the mechanism by which the first experimental gap junction agonist, rotigaptide, helps preserve gap junction communication during a heart attack and slow the onset of lethal cardiac arrhythmias. We have also begun investigating what effects novel types of anti-cancer drugs called histone deacetylase (HDAC) inhibitors have on cardiac gap junctions and the action potential they conduct. Preliminary evidence suggests that under-appreciated protein post-translational modifications like acetylation and arginylation modulate the cardiac action potential and its conduction in addition to phosphorylation. These studies involving novel protein post-translational modifications and experimental/clinical drugs are continuing in the laboratory.
- Veenstra RD and RL DeHaan. Measurement of single channel currents from cardiac gap junctions. Science 233: 972-974, 1986.
- Veenstra RD, H-Z Wang, DA Beblo, MG Chilton, AL Harris, EC Beyer, and PR Brink. Selectivity of connexin-specific gap junction channels does not correlate with channel conductance. Circ. Res. 77: 1156-1165, 1995.
- Lin X, E Fenn, and RD Veenstra. An amino terminal lysine residue of connexin40 that is required for spermine block. J. Physiol. 570: 251-269, 2006.
- Lin X, C Zemlin, J Hennan, JS Petersen, and RD Veenstra. Enhancement of ventricular gap junction coupling by rotigaptide. Cardiovasc Res 79: 416-426, 2008.
- Lin X, J Gemel, A Glass, CW Zemlin, EC Beyer, and RD Veenstra. Connexin40 and connexin43 determine gating properties of atrial gap junction channels. J Molec Cell Cardiol. 48: 238-245, 2010.
- Chen Y, Zhou Y, Lin X, Wong H-C, Xu Q, Jiang J, Wang S, Lurtz MM, Louis CF, Veenstra RD, Yang JJ. Molecular interaction and functional regulation of connexin50 gap junctions by calmodulin. Biochem J. 435: 711-722, 2011.
- Xu Q, Chen Y, Kopp R, Yang JJ, Roe MW, Veenstra RD. Calcium regulation of connexin43 via a cytoplasmic loop calmodulin binding domain gating mechanism. Am J Physiol Cell Physiol 302:C1548-C1556, 2012.
Dr. Dennis Stelzner has been elected a Fellow in the American Association of Anatomists. He was presented with a citation and plaque at the annual meeting of the American Association of Anatomists during the FASEB meeting on April 12, 2011 in Washington, DC.
The citation reads:
Spinal cord injury (SCI) has been studied during his entire career using neuroanatomical and ultrastructural methods. He showed that the ability of nerve tracts to regenerate or grow around partial SCI during development is dependent on their maturation at the time of injury.
Differences were also found in the ability of frog optic and tectal efferent axons to regenerate through the same diencephalic injury. The intrinsic cellular response needed for CNS axons to regenerate is the focus of his present work on propriospinal neurons using "molecular neuroanatomy" to determine factors underlying a maximal regenerative response after spinal cord injury.