Publications

JOURNALS

Prickett KS, Amberg DC, Hopp TP (1989) A calcium dependent antibody for identification and purification of recombinant proteins, Biotechniques, 7, 580-589.

Amberg DC, Goldstein AL, Cole CN (1992) Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA, Genes Devel., 6, 1173-1189. External Icon

Amberg DC, Fleischmann M, Stagljar I, Cole CN, Aebi M (1993) Nuclear PRP20 Protein is Required for mRNA Export, EMBO J., 12, 233-241.

Bogerd AM, Hoffman JA, Amberg DC, Fink G, Davis LI (1994) nup1 mutants exhibit pleitropic defects in nuclear pore complex function, J. Cell Biol., 127, 319-332. External Icon

Amberg DC, Basart E, Botstein D (1995) Defining Protein Interactions with Yeast Actin in vivo, Nature Structural Biology, 2, 28-35.

Li O, Heath CV, Amberg DC, Dockendorff TC, Copeland CS, Snyder M, Cole CN (1995), Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes, Mol. Biol. Cell, 6, 401-417. External Icon

Amberg DC, Botstein D, Beasley EM (1995) Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction, Yeast, 11, 1275-1280.

Heath CV, Copeland CS, Amberg DC, Del Priore V, Snyder M, Cole CN (1995) Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene, J. Cell Biol., 131, 1677-97. External Icon

Saavedra C, Tung K-S, Amberg DC, Hopper AK and Cole CN (1996) Regulation of mRNA Export in Response to Stress in Saccharomyces cerevisiae, Genes and Devel., 10, 1608-20. External Icon

Amberg DC, Zahner JE, Mulholland JW, Pringle JR and Botstein D (1997) Aip3p/Bud6p, a yeast actin-interacting protein that is involved in morphogenesis and the selection of bipolar budding sites, Mol. Biol. Cell, 8, 729-753. External Icon

Amberg DC (1998) Three-dimensional imaging of the yeast actin-cytoskeleton through the budding cell-cycle, Molec. Biol. Cell, 8, 3259-62. External Icon

Rodal A, Tetreault J, Lappalainin P, Drubin DG, and Amberg DC (1999) Aip1p Interacts with Cofilin to diassemble Actin Filaments, J. Cell Biol., 145, 1251-64. External Icon

Jin H and Amberg DC (2000) The Secretory Pathway Mediates Localization of the Cell Polarity Regulator Aip3p/Bud6p. Molec. Biol. Cell, 11, 647-661. External Icon

Jin H and Amberg DC (2001) Fission yeast Aip3p (spAip3p) is required for an alternative actin-directed polarity program. Molec. Biol. Cell, 12, 1275-1291. External Icon

Evangelista M, Pruyne D, Amberg DC, Boone C, and Bretscher A. (2002) Formins direct Arp2/3-independent actin filament assembly to polarize cell growth in yeast. Nat Cell Biol, 4(3):260-9. External Icon

Yuzyuk T, Foehr M, and Amberg, DC (2002) The MEK kinase Ssk2p promotes actin cytoskeleton recovery after osmotic stress. Mol. Biol. Cell, 13, 2869-2880. External Icon

Yuzyuk T, Foehr M, and Amberg, DC (2003) Actin recovery and bud emergence in osmotically stressed cells requires the conserved actin interacting mitogen-activated protein kinase kinase kinase Ssk2p/MTK1 and the scaffold protein Spa2p. Mol. Biol. Cell 14(7):3013-26. External Icon

Bettinger BT, Gilbert DM, and Amberg DC (2004) Actin up in the nucleus. Nat Rev Mol Cell Biol. 5(5):410-5. External Icon

Clark MG, Temply J, Haarer BK, Viggiano SC, Sept D and Amberg D (2006) A genetic dissection of Aip1p's interactions leads to a model for Aip1p-cofilin cooperative activities. Molec. Biol. Cell, 17, 1971-1984. External Icon

Daniel JA, Yoo J, Bettinger BT, Amberg DC and Burke D. (2006) Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae. Genetics, 172, 709-711. External Icon

Haarer BK, Helfant AH and Amberg D (2007) Stable pre-anaphase spindle positioning requires Bud6p/Aip3p and an apparent interaction between the spindle pole bodies and the neck. Euk. Cell, May; 6(5): 797-807. Epub 2007 Apr 6. External Icon

Farah ME and Amberg D (2007) Conserved actin cysteine residues are oxidative stress sensors that can regulate cell death in yeast. Molec. Biol. Cell, April 2007;18 (4): 359-1365. External Icon

Haarer BK,Viggiano SC, Hibbs MA, Troyanskaya OG and Amberg DC (2007) Modeling complex genetic interactions in a simple eukaryotic genome: Actin displays a rich spectrum of complex haploinsufficiencies. Genes and Devel., 21, 149-159. This article was featured on the cover and in a perspective published in the same issue. External Icon

Bettinger BT, Clark MG and Amberg DC (2007) Requirement for the polarisome and formin function in Ssk2p-mediated actin recovery form osmotic stress in Saccharomyces cerevisiae. Genetics, Apr; 175(4): 1637-48. Epub 2007 Jan 21. External Icon

Bettinger BT and Amberg DC (2007) The MEK kinases MEKK4/Ssk2p facilitate complexity in the stress signaling responses of diverse systems. J. Cell. Biochem., 2007 May 1; 101(1): 34-43 External Icon

Clark MG and Amberg DC (2007) Biochemical and genetic analyses provide insight into the structural and mechanistic properties of actin filament disassembly by the Aip1p-cofilin complex in Saccharomyces cerevisiae. Genetics, 2007 May 4; [Epub ahead of print]. External Icon

Scarcelli JJ, Viggiano S, Hodge CA, Heath CV, Amberg DC, and Cole CN. Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components. (2008) Genetics, 179: 1945-1955

Viggiano S, Haarer B, and Amberg DC. Correction/Completion of the yeast actin, alanine scan alleles. (2010) Genetics, 185: 391-394

Farah, M.E., Sirotkin, V., Haarer, B., Kakhniashvili, D., and Amberg, D.C. Diverse Protective Roles of the Actin Cytoskeleton During Oxidative Stress. (2011) Cytoskeleton, 68: 340-354

Haarer, B., Aggeli, D., Viggiano, S., Burke, D.J., and Amberg, D.C. Novel Interactions between Actin and the Proteasome Revealed by Complex Haploinsufficiency. (2011) PLOS Genetics, 7: e1002288

Haarer, B., Mi-Mi, L., Cho, J., Cortese, M., Viggiano, S., Burke, D., and Amberg, D.C. Actin Dosage Lethality Screening in Yeast Mediated by Selective Ploidy Abalation Reveals Links to Urmylation/Wobble Codon Recognition and Chromosome Stability. (2013) G3: Genes/Genomes/Genetics, 3:553-561

DiPrima S, Haarer B, Viggiano S, Pons, C, Myers CL, and Amberg DC (2014) Linking genetics to structural biology: complex heterozygosity screening with actin alanine scan alleles identifies functionally related surfaces on yeast actin. (2014) G3: Genes/Genomes/Genetics, 4:1491-501

Aggeli D, Kish-Trier E, Haarer B., Cingolani G, Wilkins S, and Amberg D.C. (2014) Coordination of the Filament Stabilizing Versus Destabilizing Activities of Cofilin Through its Secondary Binding Site on Actin. (2014) Cytoskeleton, 71:361-79

Silva, RC., Dautel, M., Di Genova, BM., Amberg, DC., Castilho, BA., and Sattlegger, E. (2015) The Gcn2 Regulator Yih1 Interacts with the Cyclin Dependent Kinase Cdc28 and Promotes Cell Cycle Progression through G2/M in Budding Yeast. PLOS One, July 15 2015

BOOKS

Amberg DC, Burke D and Strathern J. (2005) Methods in Yeast Genetics. Cold Spring Harbor Press.

BOOK CHAPTERS

Amberg DC and Botstein D (1996) Obtaining structural information about multi-protein complexes with the two-hybrid system. In "The Two-Hybrid System" (P.L. Bartel and S. Fields, eds.), pgs. 99-108, Oxford University Press, Oxford.

Botstein D, Amberg D, Mulholland J, Huffaker T, Adams A, Drubin D, and Stearns T (1997) The Yeast Cytoskeleton. In "The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology" (J.R. Pringle, J.R. Broach, and E.W. Jones, eds.), pgs. 1-90, Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

Cole CN, Heath CV, Hodge CA, Hammell CM, and Amberg DC (2002) Analysis of RNA export, Methods in Enzymology, 351:568-87.

Amberg, D, Leadsham, JE, Kotiadis, V, and Gourlay, CW. Cellular ageing and the actin cytoskeleton, Sub-cellular Biochemistry, 57:331-352 (2012)

Amberg, D.C. and Burke, D.J. Classical Genetics with Saccharomyces cerevisiae. Cold Spring Harb. Protoc. 2016