The model above was obtained using a directed
modeling approach to dock cofilin onto actin basd on the co-crystal
structure of gelsolin (has a homologous actin binding domain to
cofilin) with actin. This model could not agree more with our
two-hybrid data. We found that act1-103 (alters amino acids 334,
335, and 336), act1-106 (alters amino acids 290, 291, and 292)
and act1-126 (alters amino acids 326 and 328) could not bind to
actin in a two-hybrid assay. As can be seen above, the Wriggers
model predicts close contact between cofilin and actin residues
326 and 328 (act1-126), actin residues 291 and 292 (act1-106)
and actin residue 334 (act1-103). Note that both our and the Wriggers
models likely reflect G-actin binding but Dr. Amy McGough believes
that both F- and G-actin binding will share contact sites (personal
communication).
