Cofilin Mis-localizes in Strains Lacking
Aip1p
or Lacking Aip1p from the Cortical Patch
As can be seen below, aip1 deletion
strains have aberrant cofilin localization. Cofilin is normally
found concentrated in cortical patches and througout the cytosol.
However, in the aip1 deletion strain cofilin appears to
localize to structures that stain with anti-actin antibodies and
morphologically appear to be actin cables. Interestingly the mutant
cofilin, cof1-19p, also aberrantly localizes to these cable-like
structures. We have also found that Aip1p fails to localize to
cortical patches in a cof1-19 strain suggesting that proper cofilin
localization (restriction to cortical patches) requires that Aip1p
be localized to the cortical patches. Note that guinea pig anti-actin
antibodies and rabbit anti-cofilin antibodies were used with a
methanol/acetone fixation procedure.
