Gene Information Page

(ANC1)

Summary

Compiled with the assistance of the SGD, BioGRID, & GFP localization websites

ANC1 was identified in the primary haploid screen as CHI with act1?. It is a subunit (30 kDa) of the TFIID, TFIIF, and SWI/SNF complexes, is involved in RNA polymerase II transcription initiation and in chromatin modification, and contains a YEATS domain. Anc1p is also known as TafII30. It is part of the Ino80 complex which includes: Act1, Arp4, Arp5, Arp8, Ies1, Ies3, Ino80, Nhp10, Rvb1, Rvb2, and Taf14. Anc1p also immunoprecipitates with Rpb9, Taf2, Sap185, Mus81, Taf14, Rpb5, Rpo21, and Tfg1 and is in a purified complex with Snf5. The anc1? null shows reduced fitness in YPD and is reported to have a depolarized actin cytoskeleton (see below). Anc1p-GFP localizes exclusively to the nucleus with 3120 copies per cell as per the O’Shea GFP web site. ANC1 has no paralog but has some homology to Sas5p which is a histone acetylase that regulates transcription. Actin staining of the null haploid showed very large cells and a high percentage of cells with gross morphology defects, extremely numerous but thin cables-scored as having strong defects

Interactions specific to this study

Genetic

Gene
act1?
act1-1
act1-4
act1-105
act1-111

Method
Complex haploinsufficiency
Unlinked non-complementation
Unlinked non-complementation
Unlinked non-complementation
Unlinked non-complementation

Source
This study
Welch et al
Welch et al
This study
This study

Physical

Gene

Method

Source

Actin Staining in null: RP Staining

SGD Page
Localization
BioGRID Page
Literature